Wir benötigen Ihre Einwilligung zur Verwendung der einzelnen Daten, damit Sie unter anderem Informationen zu Ihren Interessen einsehen können. Klicken Sie auf "OK", um Ihre Zustimmung zu erteilen.
Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor
Name übersetzen
NORM herausgegeben am 1.4.2017
Bezeichnung normen: ASTM E2196-17
Anmerkung: UNGÜLTIG
Ausgabedatum normen: 1.4.2017
SKU: NS-683231
Zahl der Seiten: 6
Gewicht ca.: 18 g (0.04 Pfund)
Land: Amerikanische technische Norm
Kategorie: Technische Normen ASTM
Keywords:
analysis, biofilm, coupon, growth reactor, Pseudomonas aeruginosa, reactor, sampling,, ICS Number Code 07.100.01 (Microbiology in general)
Significance and Use | ||||
5.1 Bacteria that exist in a biofilm are phenotypically different from suspended cells of the same genotype. The study of biofilm in the laboratory requires protocols that account for this difference. Laboratory biofilms are engineered in growth reactors designed to produce a specific biofilm type. Altering system parameters will correspondingly result in a change in the biofilm. The purpose of this method is to direct a user in the laboratory study of biofilms by clearly defining each system parameter. This method will enable a person to grow, sample, and analyze a laboratory biofilm. The method was originally developed to study toilet bowl biofilms, but may also be utilized for research that requires a biofilm grown under moderate fluid shear. |
||||
1. Scope | ||||
1.1 This test method is used for growing a reproducible 1.2 Although this test method was created to mimic conditions within a toilet bowl, it can be adapted for the growth and characterization of varying species of biofilm (rotating disk reactor—repeatability and relevance (2)). 1.3 This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log10 colony forming units per surface area (rotating disk reactor—efficacy test method (1.4 Basic microbiology training is required to perform this test method. 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. |
||||
2. Referenced Documents | ||||
|
Wollen Sie sich sicher sein, dass Sie nur die gültigen technischen Normen verwenden?
Wir bieten Ihnen eine Lösung, die Ihnen eine Monatsübersicht über die Aktualität der von Ihnen angewandten Normen sicher stellt.
Brauchen Sie mehr Informationen? Sehen Sie sich diese Seite an.
Letzte Aktualisierung: 2024-04-18 (Zahl der Positionen: 2 859 866)
© Copyright 2024 NORMSERVIS s.r.o.